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KMID : 1094720190240020366
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Biotechnology and Bioprocess Engineering
2019 Volume.24 No. 2 p.366 ~ p.374
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Enzymatic and Microbial Biosynthesis of Novel Violacein Glycosides with Enhanced Water Solubility and Improved Anti-nematode Activity
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Lee Yu-Jeong
Bashyal Puspalata
Pandey Ramesh Prasad
Sohng Jae-Kyung
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Abstract
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Violacein, a microbial metabolite with multiple applications, was produced in Escherichia coli, and glycodiversified using purified Bacillus glycosyltransferase (YjiC) enzyme with glucose, galactose, and N-acetylglucosamine, to generate five novel O-glycoside derivatives. One of the glucose-conjugated derivatives, violacein 5′-O-glucoside, was produced from engineered E. coli harboring entire violacein biosynthetic gene cluster (VioABCDE) and a glycosyltransferase gene (yjiC) in tryptophan supplemented TB medium. Violacein 5′-O-glucoside gained anti-nematodal activity against pine wood nematode Bursaphelenchus xylophilus, a causative agent of pine wilt disease. Moreover, the conjugation of sugar moieties in violacein enhanced water solubility. Violacein 5′-O-diglucoside was completely retained in water fraction, while its aglycone parent molecule was completely insoluble in water.
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KEYWORD
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violacein, glycodiversification, anti-nematode, novel glycosides
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